Untargeted Metabolomics Analysis of Liquid Endosperm of Cocos nucifera L. at Three Stages of Maturation Evidenced Differences in Metabolic Regulation

Cocos nucifera L. is one of the most cultivated palm trees in world since it used to obtain both raw materials and food. From a human point view, coconut fruit very valuable product, producing an aromatic tasty liquid endosperm (coconut water) containing high levels sugars, amino acids other molecules nutritional nutraceutical value. Most chemical composition studies conducted on date have focused determination fatty acid content oil extension shelf life water. Despite economic importance this species, maturation complex biological process scarcely studied from metabolic approach biochemical changes occurring during are not well-known. The objective study investigate elucidate that occur (Cocos L.) fruits, specifically focusing Yucatan green dwarf variety. In study, fruits at immature, intermediate mature stages been analyzed through untargeted metabolomics by ultra performance chromatography coupled resolution mass spectrometry (UPLC-HRMS). A total 591 spectrometric features were detected corresponding identified compounds classified into 24 classes. principal component analysis (PCA) showed segregation among samples, according their stage maturation. metabolites related metabolism flavonoids, carbohydrates organooxygen compounds. Pathway sphingolipid, starch sucrose metabolisms over-accumulated ripening, followed glyoxylates dicarboxylates such as alanine, aspartate glutamate, others. This first focuses elucidating profiles variety three with UPLC-MS.